We host a Google forum where scientists from institutions across the world can find answers to their questions about CRISPR usage in laboratory settings. Questions posted on the site are viewed and answered by members of the Zhang lab.
We recently shut down our guide design web tool, but we have compiled some resources that may be helpful if you need assistance with guide design.
Each year, we host a workshop at the Broad Institute open to anyone who wants to learn more about genome engineering and get one-on-one advice about implementing CRISPR-mediated tools in their research.
Information and protocols for high-throughput loss-of-function screening with Cas9 can be found on the Sanjana lab webiste.
Interested in RNA targeting? We have written a primer on using Cas13.
You can obtain reagents via the nonprofit plasmid repository Addgene. There is also information available there about protocols for working with these tools.
If you cannot obtain reagents through Addgene, please feel free to reach out to us directly