Genome Engineering Workshop

Each year, the Zhang lab hosts a two-day workshop aimed at sharing knowledge about CRISPR-mediated tools and other aspects of genome engineering, from basic biology to technology development. The workshop is geared toward scientists at all levels of training who have an interest in learning more about implementing genome engineering approaches in their own work and in keeping up with the latest developments in this field.

Thank you to everyone who attended our seventh annual Genome Engineering Workshop! Videos of the primers from the first day of the workshop are now available.

CRISPR basics (Linyi Gao, Zhang Lab)

CRISPR Screens (Julia Joung, Zhang Lab)

RNA-targeting with CRISPR (Soumya Kannan, Zhang Lab)


CRISPR in vivo

May 19 & 20, 2019

Broad Institute

cambridge ma


CRISPR in vivo

The 2019 workshop will take a closer look at applications of genome engineering for human therapeutics, including talks about improving the specificity and efficiency of gene editing, delivery of molecular therapeutics, gene therapy and immunity, and exploring natural diversity to find new potential tools. The workshop will begin Sunday, May 19th at 1:00 and conclude Monday, May 20th at 5:00.


8:30 – 9:00 Registration

9:00 – 9:45 – Keynote: Keith Joung, MGH and Harvard

                        Defining and improving the specificities of CRISPR-guided DNA base editors

9:45 – 10:05 – Eugene Koonin, NCBI

                        Evolutionary diversification of CRISPR-Cas: far beyond adaptive immunity

10:05 – 10:25 – David Paez-Espino, JGI

                          Metagenomic Class2 Cas genes and their connection with phage

10:25 – 10:55 – Coffee break

10:55 – 11:15 – Jonathan Strecker, Zhang lab at Broad Institute

                        Genome editing with Type V CRISPR systems

11:15 – 11:35 – Bin Gu, Rossant lab at Sick Kids

                          Light up the embryos: Efficient generation of knock-in report mice by 2C-HR-CRISPR

11:35 – 11:55 – Jonathan Wilde, Feng lab at MIT

                          Efficient Embryonic Genome Editing via RAD51-Enhanced Interhomolog Repair

11:55 – 12:15 – Sukanya Iyer, Wolfe lab at UMass Medical

                         Precise therapeutic gene correction by a simple nuclease-induced double-stranded break

12:15 – 1:00 – Lunch

1:00 – 1:20 – Luke Koblan & Jon Levy, Liu lab at Broad Institute

                        In vivo Correction of Pathogenic SNPs with Optimized Base Editors

1:20 – 1:45 – Gail Mandel (OHSU)

                        Application of RNA editing to curing the neurological disease, Rett syndrome: updates

1:45 – 2:05 – Jonathan Gootenberg (Zhang lab at Broad Institute)

                        Extending the range of RNA editing with CRISPR-Cas13

2:05 – 2:25 – Shirley Liu, Dana Farber Cancer Institute and Harvard,

Integrating CRISPR screens and computational analyses to identify drugs with immune modulatory effects

2:25 – 2:55 – Coffee

2:55 – 3:15 – Arlene Sharpe, Harvard

CRISPR-based screening in the hematopoietic system to identify novel regulators of antimicrobial immunity

3:15 – 3:35 – Jorge Mansilla-Soto, Sadelain lab at Memorial Sloan Kettering Cancer Center

                        Advancing CAR therapy with precise T cell genome engineering

3:35 – 3:55 – James Dahlman, GeorgiaTech

Improving RNA delivery by testing thousands of nanoparticles in vivo using DNA barcodes

3:55 – 4:15 – Eric Zinn, Vandenberghe lab at Harvard

Decoding AAV through in silico design and multi-parametric screening

4:15 – 5:00 – Keynote: Katrine Bosley

                        The Path to CRISPR-based therapeutics


12:30 – 1:00 Registration & Poster hanging

1:00 – 1:45  CRISPR basics Linyi Gao, Zhang Lab

1:45 – 2:15 CRISPR screens Julia Joung, Zhang Lab

2:15 – 2:45  RNA-targeting with CRISPR Soumya Kannan, Zhang Lab

2:45 – 2:50 Lightning talk Benchling

2:50 – 3:10 Coffee break

3:10 – 4:00 Breakout sessions (concurrently)

  • Cas9 & Cas12 Applications Zhang lab members

  • Creating Mouse Models Lin Wu, Director of Genome Modification Facility at Harvard

  • Cas13 Applications Zhang lab members

  • CRISPR Screens Zhang lab members

  • Unlocking Endogenous Biology through CRISPR-Mediated Protein Tagging with the Luminescent Peptide HiBiT Promega

4:10 – 4:30 – Feng Zhang

4:30 – 6:30 – Happy hour & poster session


Get advice

Get one-on-one advice about your experiments from experienced CRISPR users during our breakout sessions


share your work

Present your work during the Sunday poster session and get feedback on your work and ideas for future directions


Learn the basics

The workshop will kick off on Sunday afternoon with presentations from members of the Zhang lab and other experts about working with CRISPR-based tools in the lab


Hear about new advances

Monday will feature talks from leaders in the field focused on outstanding challenges in genome engineering

Can I present my work?

You are encouraged to share your work with your fellow workshop attendees. For this purpose we will have a poster session on Sunday, May 19th. Abstracts can be submitted via the registration link or email your abstract to by April 29th.

Do I need to pay for anything?

Registration for the full workshop is $25 for academic attendees and $100 for industry attendees. This money helps cover the cost of meals served during the workshop. The rest of the event is made possible by the generosity of the Broad Institute and our sponsors.

Where can I stay?

We do not offer lodging, so you will need to make our own arrangements. There are several nearby hotels - two Marriotts and the Kendall Hotel. The Broad is right off the Red Line subway at the Kendall/MIT stop, so anyplace that is near the Red Line will also be convenient for getting to the meeting.


Thank you to our Sponsors for helping support this workshop

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