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Guide Design Resources

Thank you to the thousands of users who visited our guide design tool over the past five years. We recently shut down crispr.mit.edu, but there are many other guide design tools available that we hope you will find helpful.

 

Tools for Guide design

Benchling

Broad Institute GPP

CasOFFinder

CHOPCHOP

CRISPOR

Deskgen

E-CRISP

Geneious (not cloud based)

Guides (for library design)

IDT

Synthego

 

Advice on designing guides

Addgene blog post

Comprehensive list of guide design tools

Wiki on Cas9 guide design